:蛋白染料及定量檢測試劑盒

產品介紹

蛋白凝膠電泳及相關的Western-Blot是蛋白分析的主流技術 (Figure 1)。GeneCopoeia公司提供兩種蛋白染料: 1) Coomassie® Blue Fast Stain, 一種即用型的蛋白染料,無需甲醇或乙酸固定、或脫色; 2) eLuminol™ Protein Gel Stain,一種具有極高靈敏度的蛋白熒光染料,用于檢測SDS-PAGE凝膠電泳中的總蛋白。GeneCopoeia提供的 ECL Western Blotting Detection Kit用于WB檢測HRP標記的抗體及其關聯的抗原,具有快速檢測、靈敏度極高等特點。另外,GeneCopoeia 也提供了兩種蛋白定量分析試劑盒: 1) Bradford Protein Assay Kit;和 2) BCA Protein Assay Kit。

Figure 1. Protein gel analysis workflow

蛋白染料選購指南

  Coomassie® Blue Fast Stain eLuminol™ Protein Gel Stain
靈敏度 20 ng 0.5 ng
操作性 Ready to use Supplied as stock solution
耗時 2 hr (standard)
20 min (microwave)
90 min (standard)
30 min (microwave)
檢測方法 比色法 熒光
質譜兼容

 

Coomassie  

Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.

ECL化學發光檢測試劑盒

                            

Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).

                  Bradford Protein Assay Kit BCA Protein Assay Kit

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).

 

訂購

Buy 貨號 產品名稱 描述 規格 價格(¥)
P001 Coomassie® Blue Fast Stain Colorimetric protein gel stain 500 mL 300

P003A
P003B
eLuminol™ Protein Gel Stain (1,000X) Fluorescent protein gel stain 500 µL
1 mL
600
1000
P007 ECL Western Blotting Detection Kit Western blotting reagent 100 mL 400
P010 Bradford Protein Assay Kit Protein quantitation 1000 assays 350
P011 BCA Protein Assay Kit Protein quantitation 1000 assays 500
P013 SDS-PAGE Sample Loading Buffer (5X) Loading buffer 5 mL 70